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Image Search Results
Journal: medRxiv
Article Title: Development and Validation of a Mobile Laboratory Workflows for Wastewater and Environmental Surveillance with Application in Sub Saharan Africa
doi: 10.64898/2026.04.01.26349919
Figure Lengend Snippet: Benchmarking of Multiplex PCR metabarcoding and ONT-16S Barcoding kit. ( A ) Microbe community composition obtained from ONT-16S (left panels) and Multiplex PCR metabarcoding (right panels) sequencing of ML-extracted (ML) and ZymoBIOMICS-extracted (ZP) samples. ( B ) Venn diagrams depicting unique and shared bacterial taxa across extraction methods and sequencing approaches. The diagram shows the percentage of bacterial features detected with >1 read in all three replicates for the two extraction methods: ML (mobile laboratory workflow) and ZP (ZymoBIOMICS kit). Left: Oxford Nanopore 16S kit; Right: library preparation using a multiplex primer set. ( C ) Venn diagrams of bacterial features shared between library preparation methods. Features with ≥1 read in all three replicates are shown for the 16S kit (Oxford Nanopore) and the Multiplex primer set. Left: Mobile laboratory (ML) protocol; Right: ZymoBIOMICS (ZP) protocol
Article Snippet: DNA was extracted from 24h composite wastewater samples (after prior filtration through 0.22 μm nitrocellulose membranes, WhatmanTM 25 mm) and the
Techniques: Multiplex Assay, Sequencing, Extraction